Apparatus, methods and processes for sorting particles and for providing sex-sorted animal sperm

Abstract From the Canadian patent

A process for evaluating a set of conditions for staining a population of cells for sorting the population comprising a first type and a second type of cell the process comprising: (a) staining a fraction of the population of cells with a fluorescent dye under a set of staining conditions; (b) exposing the stained cells to electromagnetic radiation as the stained cells are passed through an interrogation location of a flow cytometer at a rate R; (c) determining a fluorescence emission characteristic of the exposed cells; (d) using the determined fluorescence characteristic to classify the exposed cells into two or more sub-populations of cells one of the sub-populations being an enriched …