Multiple-color monochromatic light absorption and quantification of light absorption in a stained sample

Abstract From the Canadian patent

A method is disclosed for quantifying light absorption in a sample using a system that includes a source of light a first detector and a second detector. The method includes impinging a beam of light on the sample measuring an amount of light loss due to interference of the beam by the sample with the first detector and producing a first signal. The method further includes measuring an amount of fluorescence emitted by the sample with the second detector and producing a second signal. The method further includes using the second signal to correct the first signal in order to quantify the amount of light loss due to a dye in the sample.