Methods for generating high titer helper-free preparations of recombinant aav vectors

Abstract From the Canadian patent

This invention provides methods and compositions for producing high titer substantially purified preparations of recombinant adeno-associated virus (AAV) that can be used as vectors for gene delivery. At the onset of vector production AAV producer cells of this invention typically comprise one or more AAV packaging genes an AAV vector comprising a heterelogous (i.e. non-AAV) transgene of interest and a helper virus such as an adenovirus. The AAV vector preparations produced are generally replication incompetent but are capable of mediating delivery of a transgene of interest (such as a therapeutic gene) to any of a wide variety of tissues and cells. The AAV vector preparations produced …