Message abundance and allele copy number determination using ivt with single-stranded primer-promoter-selector constructs

Abstract From the Canadian patent

A single stranded primer-promoter-selector construct for detecting the presence or the relative , quantity of a target subsequence in a sample. The construct includes (in 3' to 5' orientation) a primer , subsequence complimentary to the target a T7 or other promoter subsequence, and a selector subsequence. The primer can be extended by template mediated elongation including reverse , transcription, or by ligation to an oligonucleotide. The promoter is oriented to direct in-vitro transcription , (IVT) opposite to that of primer extension where the selector serves as a , template for IVT. The selector is associated with the target of interest and it and the amplified product, are , …