Treatment of bioprosthetic tissues to mitigate post implantation calcification

Abstract From the Canadian patent

The present invention provides methods for treating tissue to inhibit postimplant calcification of a biological tissue. In one method of this invention a tissue is immersed in or otherwise contacted with a pretreated glutaraldehyde solution, i.e. a heat-treated or pH-adjusted glutaraldehyde solution. The tissue may be partially fixed with glutaraldehyde prior to after, or concurrently with the step of contacting the tissue with the pretreated gluteraldehyde. Contact with the pretreated gluteraldehyde produces free amine groups on the tissue, which are subsequently blocked by contacting the crosslinked tissue with a blocking agent. In another embodiment a tissue , is contacted with …