Method for quantitative and/or comparative measurement of mrna expression levels in small biological samples
CA 2568537 C · Owner: Stenman, Jakob · Inventors: Stenman, Jakob; Orpana, Arto; Paju, Annukka
Overview
Its claims, description and drawings are in the Canadian Patents Database.
Open the CIPO recordAbstract From the Canadian patent
The present invention provides a method for quantitative and/or comparative assessment of the relative amounts of mRNA transcripts present in a cell or tissue sample. In the method reverse transcription of the mRNA contained in the sample is first carried out using sequence-modifying primers for one or several genes in the same reaction to obtain a pool of sequence-modified cDNA molecules. After completion of the reverse transcription redundant sequencemodifying primers are removed or inactivated. This step is followed by a step of co-amplifying the sequence-modified cDNA templates with a reference DNA template in individual gene-specific amplification reactions. By quantitatively …
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Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Filed May 25, 2005 and granted April 2, 2013. A Canadian patent filed from October 1, 1989 lasts 20 years from filing, so its term ended May 25, 2025.
- Certificates of supplementary protection extend only drug patents, and it isn't classified as one.
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Timeline
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Official CIPO records
Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.