Oligopeptide ligation methods comprising an activated ester moiety or an aldehyde or ketone moiety

Abstract From the Canadian patent

A method of ligating two or more molecules for example, peptides to peptides , or peptides to labels is provided. The method may comprise the steps: a) providing a first oligopeptide having a reactive moiety which is a hydrazine , moiety, a hydrazide moiety or an amino-óxy moiety b) providing a second oligopeptide having an activated ester moiety c)allowing the reactive moiety , of the first oligopeptide to react with the activated ester moiety of the second oligopeptide to form an oligopeptide product in which the first and , second oligopeptides are linked via a linking moiety having Formula I II or , III. The second oligopeptide may preferably be generated by thiol reagent induced …