Methods for expression and purification of immunotoxins
CA 2534360 C · Owner: The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services, National Institutes Of Health · Inventors: Neville, David M.; Liu, Yuan-Yi; Woo, Jung-Hee
Overview
Its claims, description and drawings are in the Canadian Patents Database.
Open the CIPO recordAbstract From the Canadian patent
In one aspect the present invention relates to a method of expressing an immunotoxin in Pichia pastoris strain mutated to toxin resistance comprising a) growing the Pichia pastoris in a growth medium comprising an enzymatic digest of protein and yeast extract and maintaing a dissolved oxygen concentration at 40% and above; and b) performing methanol induction with a limited methanol feed of 0.5-0.75 ml/min/ 10L of initial volume during induction along with a continuous inusion of yeast extract at a temperature below 17.5 ~C. antifoaming agent supplied up to 0.07% agitation reduced to , 400 RPM, and the induction phase extended out to 163 h. In another aspect the , present invention …
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Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Filed August 2, 2004 and granted January 28, 2014. A Canadian patent filed from October 1, 1989 lasts 20 years from filing, so its term ended August 2, 2024.
- A certificate of supplementary protection adds at most two years for a drug, so even that would have ended by August 2, 2026.
- We check Canadian rights only. A patent in its family in another country could still be in force: check its family on Google Patents.
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Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.