Quantitative pcr method of detecting specific plant genus in food or food ingredient

Abstract From the Canadian patent

Provided is a method of quantifying a specific plant genus in a food or a food ingredient by a PCR method, comprising: (i) preparing a sample for correction where a sample, derived from the specific plant genus to be detected and a standard plant sample are mixed in a, predetermined ratio and extracting genomic DNA from the sample for correction; (ii), preparing a test sample where a known amount of the standard plant sample is added to the, food or the food ingredient to be examined and extracting genomic DNA from , the test sample; (iii) practicing a quantitative PCR method using the genomic DNAs and primers; and, (iv) conducting correction with a standard value for correction …