Rolling circle amplification of rna

Abstract From the Canadian patent

Disclosed are compositions and methods for amplification of RNA molecules. The disclosed method involves synthesizing first strand cDNA molecules from RNA molecules, circularizing the first strand cDNA molecules and replicating the circularized first strand cDNA molecules using rolling circle replication. The method can be aided by the use of specialized primers for cDNA synthesis and specialized probes for circularizing the first strand cDNA molecules. The method can be used to replicate and amplify multiple RNA molecules such as , all RNA molecules in a sample or all mRNA molecules in a sample or be used to , replicate and amplify specific RNA molecules. Rolling circle replication …