High sensitivity quantitation of peptides by mass spectrometry

Abstract From the Canadian patent

The instant invention provides an economical flow-through method for determining amount of target proteins in a sample. An antibody preparation (whether polyclonal or monoclonal or any equivalent specific , binding agent) is used to capture and thus enrich a specific monitor peptide (a , specific peptide fragment of a protein to be quantitated in a proteolytic digest of , a complex protein sample) and an internal standard peptide (the same chemical structure but including stable isotope labels). Upon elution into a suitable mass spectrometer, the natural (sample derived) and internal standard (isotope labeled) peptides are quantitated and their measured abundance ratio , used to …