Microarrays having multiple oligonucleotides in single array features

Abstract From the Canadian patent

The present invention is a method for synthesizing microarrays having different oligonucleotides present within one feature area of the array. The method utilizes the techniques common to microarray synthesis but limits the , duration in which selected feature areas on the array are initially dosed with light so as to only deprotect a calculated ratio of the compounds forming the array's binding layer. The compounds initially deprotected are capped with a non~photosensitive protecting group, such as di-methoxy-trityl to inhibit , their involvement in the synthesis of a first group of DNA strands built onto the array. Once the first group of DNA strands have been synthesized the , …