Method for gene isolation by cre-trap cloning
CA 2496813 A1 · Owner: Sleckman, Barry P. · Inventors: Sleckman, Barry P.
Overview
Its claims, description and drawings are in the Canadian Patents Database.
Open the CIPO recordAbstract From the Canadian patent
The disclosure describes a method for isolating genes encoding proteins that regulate the expression of target genes of interest said method designated , herein for convenience by the term: "Cre-Trap cloning." In brief the Cre-trap , cloning method of the invention involves modifying a target gene of interest such that it encodes the Cre recombinase and Herpes simplex virus (HSV) thymidine kinase (TK) proteins. This step is followed by mutagenesis and selection for cells which have lost target gene expression by virtue of their resistance to ganciclovir (a nucleoside analog structurally related to acyclovir), which kills cells expressing HSV TK. Cell lines that have lost target gene …
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Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Filed August 26, 2003. No grant is recorded for it in Canada; had it been granted, its 20-year term would have ended August 26, 2023.
- A certificate of supplementary protection adds at most two years for a drug, so even that would have ended by August 26, 2025.
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Timeline
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Official CIPO records
Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.