Circular nucleic acid vectors, and methods for making and using the same

Abstract From the Canadian patent

Circular nucleic acid vectors that provide for persistently high levels of protein expression are provided. The circular vectors of the subject invention are characterized by being devoid of expression-silencing bacterial sequences where in many embodiments the subject vectors include a unidirectional sitespecific recombination product hybrid sequence in addition to an expression cassette. Also provided are methods of using the subject vectors for introduction of a nucleic acid, e.g. an expression cassette into a target , cell, as well as preparations for use in practicing such methods. The subject methods and compositions find use in a variety of different applications including both …