Method for the characterisation of nucleic acid molecules
CA 2490949 A1 · Owner: University College Cork-National University Of Ireland, Cork · Inventors: Mccarthy, Thomas Valentine; Vaughan, Patrick Martin
Overview
Its claims, description and drawings are in the Canadian Patents Database.
Open the CIPO recordAbstract From the Canadian patent
A method for characterising nucleic acid molecules comprises the steps of i) introducing a modified base which is a substrate for a DNA endonuclease for , example Endonuclease V from E. coli into a target DNA molecule; and ii) , reacting the nucleic acid containing the modified base with said DNA endonuclease such that the nucleic acid is cleaved to generate an upstream fragment condition said modified base and bearing a 3' hydroxyl group. The upstream fragment generated in step ii) can be used as a primer for a subsequent extension reaction. The modified base as such targets cleavage of the nucleic acid strand because it directs the endonuclease to cut the nucleic acid strand on …
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Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Filed June 25, 2003. No grant is recorded for it in Canada; had it been granted, its 20-year term would have ended June 25, 2023.
- A certificate of supplementary protection adds at most two years for a drug, so even that would have ended by June 25, 2025.
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Official CIPO records
Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.