Increased delivery of a nucleic acid construct in vivo by the poly-l-glutamate (plg) system

Abstract From the Canadian patent

Plasmid DNA delivered by injection / electroporation to the skeletal muscle can be expressed, and physiologic levels of transgene could be achieved into the circulation. Nevertheless, stabilization of naked DNA may be required and necessary in some cases, as prolonged storage at different temperatures before usage, injection into a large number of animals etc. It is imperative that , the associated compound should not be toxic to the cells (e.g. muscle cells) or cause breakage of plasmid DNA. It would be preferable for the coated DNA to have a similar or increased uptake into the target cells. Low molecular weight poly~L-glutamate compounds have all the desired properties. It was …