Electroporation method including the use of depressurization/pressurization
CA 2483524 A1 · Owner: National Institute Of Agrobiological Sciences · Inventors: Tabei, Yutaka; Hagio, Takashi
Overview
Its claims, description and drawings are in the Canadian Patents Database.
Open the CIPO recordAbstract From the Canadian patent
An object of the present invention is to establish a transformation method simpler and more rapid than conventional techniques. This object can be solved by a method comprising the steps of a) holding a cell under a pressure different from an atmospheric pressure and b) placing the cell and a nucleic acid under conditions capable of inducing electroporation. The present invention removes the necessity of culture which is conventionally required after gene transfer. Therefore, the present invention makes it possible to obtain a transformant of species which are impossible or considerably difficult to transform by conventional techniques. The simple transformation method of the …
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Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Filed July 14, 2003. No grant is recorded for it in Canada; had it been granted, its 20-year term would have ended July 14, 2023.
- A certificate of supplementary protection adds at most two years for a drug, so even that would have ended by July 14, 2025.
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Timeline
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Sources & verification
Official CIPO records
Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.