Reaction solution for cell-free protein synthesis, method of preparing the same and protein synthesis method using the same

Abstract From the Canadian patent

It is intended to provide a reaction solution for cell-free protein synthesis which is suitable for folding a protein having a weakly reduced state and a , cell-free protein synthesis method characterized by performing a weakly reduced synthesis reaction, preferably by adding a substance catalyzing a disulfide bond exchange reaction at the early stage of a translation reaction and then further continuing the translation reaction. According to this method, a protein having a properly formed disulfide bond in its molecule and being comparable in function to the original one can be provided at a high efficiency. More specifically speaking an antibody protein binding , specifically to an …