Methods for two-dimensional conformationdependent separation of non-circular nucleic acids

Abstract From the Canadian patent

Methods are provided for separating linear nucleic acid fragments based on their conformation. The methods are based on novel two-dimensional gel electrophoresis techniques. In one aspect the method comprises electrophoresing a sample of nucleic acids in a first dimension said sample through a gel matrix under a first set of pre-determined electrophoresis conditions; electrophoresing said gel matrix in a second dimension under a second set of electrophoresis conditions such that linear nucleic acid , fragments of equal length but having different conformation are separated; said first and second electrophoresis conditions are different such that in , one of said dimensions …