Protein tag comprising a biotinylation domain and method for increasing solubility and determining folding state
CA 2474457 C · Owner: Dyson, Michael Richard · Inventors: Hart, Darren James; Samaddar, Mitali; Dyson, Michael Richard
Overview
Its claims, description and drawings are in the Canadian Patents Database.
Open the CIPO recordAbstract From the Canadian patent
The use of a tag moiety comprising a biotinylation domain such as biotin , carboxyl carrier protein (BCCP) as a protein folding marker and protein solubility enhancer in the orientated surface capture of products of heterologously expressed genes is described. Methods for increasing the solubility of proteins and determining the folded state of a protein are also disclosed. The uses and methods of the invention can be carried out in a multiplexed manner on more than one protein in the formation of libraries. In addition the nucleic acid molecule encoding the biotinylation domain of the tag moiety can be used to increase the proportion of clones in a library that express the protein …
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Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Filed January 29, 2003 and granted April 15, 2014. A Canadian patent filed from October 1, 1989 lasts 20 years from filing, so its term ended January 29, 2023.
- A certificate of supplementary protection adds at most two years for a drug, so even that would have ended by January 29, 2025.
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Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.