Multiply-substituted protease variants

Abstract From the Canadian patent

Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases in , general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease such as altered wash , performance. The substituted amino acid residue equivalent to positions 7 23, 26, 28, 29, 30, 31, 47, 66, 69, 73 82, 85, 88, 90, 92, 93, 105, 113, 139, 148 149, 150, …