Method and reagent of colorimetry employing an oxidoreductase and a transition metal complex that changes colour by transferring an electron

Abstract From the Canadian patent

The present invention provides a colorimetric method that can perform a simple and reliable analysis in a short time. An aqueous solution including a bipyridyl copper complex and glucose dehydrogenase is prepared. A filter paper is impregnated with the aqueous solution and dried. When a sample such as blood is applied to the filter paper the , bipyridyl copper complex produces reddish brown color in accordance with the glucose concentration, and the color produced in the complex is measured. This reaction is a single reaction and thus occurs in a short time (e.g. 5 seconds or less). Since this reaction requires neither hydrogen peroxide nor oxygen, the measured values are highly …