Process for the purification and/or isolation of biologically active granulocyte colony stimulating factor

Abstract From the Canadian patent

The invention relates to the process for the isolation of biologically active granulocyte colony stimulating factor (G-CSF) which enables the separation of , correctly folded biologically active monomeric molecules of G-CSF from the incorrectly folded, biologically inactive monomeric oligo- or polymeric and , also from aggregated molecules of G-CSF by using immobilised metal affinity chromatography. The process of the invention if desired the whole process, can be advantageously performed under native conditions. The biologically active G-CSF with a purity of greater than 95% is thus obtained. Only two additional chromatographic steps, cationic exchange chromatography and gel …