Isolation tool for viable c-kit expressing cells

Abstract From the Canadian patent

The present invention provides a tool for the isolation of ck-it expressing cells. This tool consists of a c-kit plasmid targeting vector which is capable of integrating into the wild type c-kit allele and encodes a chimeric fluorescent protein comprising a nucleolar localisation signal such as TCOF-, 12, RLP313, RPS254 or Fxr2h5.. Said construct generates a condensed bright, fluorescent signal that can be localised in living tissue and after dissociation. The construct allows visualisation using confocal microscopy and allows automated cell sorting of the dissociated cells using amongst others flow cytometry.