Methylated heparin-binding hemagglutinin recombinant mycobacterial antigen, preparation processes and immunogene compositions containing such an antigen

Abstract From the Canadian patent

The invention concerns a methylated immunogenic recombinant peptide sequence comprising mycobacterial heparin-binding hemagglutinin. The invention also concerns chemical and enzymatic methods for preparing such a sequence said sequence being previously produced in a non-methylated recombinant form then methylated by post-translational modification. The invention further concerns recombinant tools vectors and host cells for implementing post-translational enzymatic methylation of the recombinant HBHA. The invention finally concerns immunogenic compositions comprising methylated native or recombinant HBHA, such compositions being useful for preparing vaccines against mycobacterial infections.