Methods and compositions for rnai mediated inhibition of gene expression in mammals

Abstract From the Canadian patent

Methods and compositions are provided for modulating e.g. reducing, coding , sequence expression in mammals. In the subject methods an effective amount of , an RNAi agent e.g. an interfering ribonucleic acid (such as an siRNA or shRNA) or a transcription template thereof e.g. a DNA encoding an shRNA, is , administered to a non-embryonic mammal e.g. via a hydrodynamic , administration protocol. Also provided are RNAi agent pharmaceutical preparations for use in the subject methods. The subject methods and compositions find use in a variety of different applications including , academic and therapeutic applications.