Methods and compositions for highly effective transformation of filamentous fungi
CA 2452183 C · Owner: Romaine, C. Peter · Inventors: Chen, Xi; Romaine, C. Peter
Overview
Its claims, description and drawings are in the Canadian Patents Database.
Open the CIPO recordAbstract From the Canadian patent
Applicants have devised a highly effective convenient, and expeditious , genetic transformation system for filamentous fungi such as Agaricus , bisporus. The preferred method uses an Agrobacterium-mediated transformation protocol. The critical features of this protocol include co-cultivation of the bacterium with fruit body tissue instead of spores. In a preferred embodiment even higher transformation efficiencies were observed with the use of a homologous promoter in the polynucleotide expression construct in order to drive gene expression.
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Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Filed June 28, 2001 and granted December 24, 2013. A Canadian patent filed from October 1, 1989 lasts 20 years from filing, so its term ended June 28, 2021.
- A certificate of supplementary protection adds at most two years for a drug, so even that would have ended by June 28, 2023.
- We check Canadian rights only. A patent in its family in another country could still be in force: check its family on Google Patents.
Timeline
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Sources & verification
Official CIPO records
Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.