Quinacridone labelling reagents for fluorescence detection of biological materials

Abstract From the Canadian patent

Disclosed are new quinacridone dye derivatives having characteristic fluorescence lifetimes. Also disclosed are methods for labelling target biological materials employing the quinacridone dyes and use of the labelled materials in biological assays. The quinacridone derivatives have the structure (I), in which Z1 and Z2 independently represent the atoms necessary to complete one ring, two fused ring, or three fused ring aromatic or heteroaromatic systems, each ring having five or six atoms selected from carbon atoms and optionally no more than two atoms selected from oxygen nitrogen and sulphur; R3, R4, R5, R6, R7 and R8 are independently selected from hydrogen, halogen, amide, …