Acridone derivatives as labels for fluorescence detection of target materials

Abstract From the Canadian patent

Disclosed are new acridone dye derivatives having characteristic fluorescence lifetimes. Also disclosed are methods for labelling target biological materials employing the acridone dyes and use of the labelled materials in biological assays. The acridone derivatives have the following structure:in which Z1 and Z2 represent the atoms necessary to complete one ring two fused , ring, or three fused ring aromatic or heteroaromatic systems each ring having , five or six atoms selected from carbon atoms and optionally no more than two atoms selected from oxygen, nitrogen and sulphur; R2 R3, R4 and R5 are , selected from hydrogen halogen, amide, hydroxyl, cyano, nitro mono- or di-, …