Compositions and methods for the identification of protein interactions in vertebrate cells
CA 2444857 A1 · Owner: President And Fellows Of Harvard College · Inventors: Yang, Annie; Mckeon, Frank
Overview
Its claims, description and drawings are in the Canadian Patents Database.
Open the CIPO recordAbstract From the Canadian patent
The present invention relates to a two-hybrid system for studying proteinprotein interactions in mammalian host cells. The invention provides methods reagents and kits for carrying out the two-hybrid screen. Accordingly the invention provides a bait construct that is capable of targeting a bait protein to a specific subcellular locale within the host cell. The invention also provides a prey construct that contains a detection sequence fused to a prey protein. The bait and prey constructs are introduced into a host cell under conditions that promote expression of the bait and prey constructs. A positive bait/prey interaction can be detected by comparing the subcellular localization of …
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Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Filed April 22, 2002. No grant is recorded for it in Canada; had it been granted, its 20-year term would have ended April 22, 2022.
- A certificate of supplementary protection adds at most two years for a drug, so even that would have ended by April 22, 2024.
- We check Canadian rights only. A patent in its family in another country could still be in force: check its family on Google Patents.
Timeline
Timeline From CIPO dates
Sources & verification
Official CIPO records
Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.