Methods and kits useful for the simplification of complex peptide mixtures

Abstract From the Canadian patent

Methods and reagents for obtaining simplified mixtures of peptides from a sample containing a number of peptides are disclosed. The simplified sample can be easier to analyze than the original peptide sample yet it is representative of all or nearly all of the proteins present in the mixed protein sample from which the original and more complex peptide sample was derived. The methods entail the use of tagging moieties that include an aminoacid-specific reactive group (R). The tagging moieties "tag" peptides or proteins at specific amino acids (e.g. by reacting with an amino acid to form a covalent bond), ultimately allowing the isolation of peptides that contain those specific amino …