5'-thio phosphate directed ligation of oligonucleotides and use in detection of single nucleotide polymorphisms
CA 2433634 A1 · Owner: Bandaru, Rajanikanth · Inventors: Bandaru, Rajanikanth; Kumar, Gyanendra
Overview
Its claims, description and drawings are in the Canadian Patents Database.
Open the CIPO recordAbstract From the Canadian patent
The present invention provides a novel method for ligation of oligonucleotides containing 5'-phosphorothioates on complementary templates by the action of DNA ligases. This reaction is readily applied to the synthesis of a single stranded circular DNA containing a phosphorothioate directed ligation reaction by ATP dependent DNA ligase reaction is similar to conventional 5'-phosphate ligation. The utility of enzymatic ligation in probing specific sequences of DNA is also described. The present invention also provides a novel nonenzymatic ligation of 5'-phosphorothioates that has been applied to the synthesis of single strand phosphorothioate and phosphate circular DNA. A process for …
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Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Filed January 4, 2002. No grant is recorded for it in Canada; had it been granted, its 20-year term would have ended January 4, 2022.
- A certificate of supplementary protection adds at most two years for a drug, so even that would have ended by January 4, 2024.
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Timeline
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Official CIPO records
Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.