Method of modifying peptide synthetases such that they can n-methylate their substrate amino acids

Abstract From the Canadian patent

The invention relates to a method of modifying peptide synthetases in such a manner that they can N-methylate their substrate amino acids. PPS are enzymes that synthesize peptides in a non-ribosomal manner. PPS have a modular set-up. Each module has an activation domain which recognizes and covalently binds to the respective substrate amino acid. The peptide synthesis catalyzed by PPS proceeds by the condensation of the covalently bound substrate amino acids. A minor number of the known activation domains is capable of also N-methylating the bound substrate amino acids. The inventive method allows conversion of the activation domains without N-methyl transferase activity to …