Screening method for the discovery and directed evolution of oxygenase enzymes
CA 2400660 A1 · Owner: Arnold, Frances F. · Inventors: Joern, John; Sakamoto, Takeshi; Arnold, Frances F.
Overview
Its claims, description and drawings are in the Canadian Patents Database.
Open the CIPO recordAbstract From the Canadian patent
The present invention relates to screening methods for oxidation enzymes particularly mono- and dioxygenases. According to the methods of the invention, a product of an oxidation reaction is converted into a phenol or a catechol, which is easily detected by a Gibbs assay. This conversion allows for a sensitive and efficient assay. Both high-throughput liquid-phase and solid-phase methods using these principles are provided. Also described are methods for detecting phenolic ether-products and sulfhydryl products from oxidation reactions, also using a Gibbs assay. Figure (1) shows this athway for a generic, monosubstituted benzene being degraded by the toluene dioxygenase system.
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Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Filed April 5, 2001. No grant is recorded for it in Canada; had it been granted, its 20-year term would have ended April 5, 2021.
- A certificate of supplementary protection adds at most two years for a drug, so even that would have ended by April 5, 2023.
- We check Canadian rights only. A patent in its family in another country could still be in force: check its family on Google Patents.
Timeline
Timeline From CIPO dates
Sources & verification
Official CIPO records
Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.