Pyrophosphorolysis activated polymerization (pap): application to allele-specific amplification and nucleic acid sequence determination
CA 2395391 C · Owner: Sommer, Steve S. · Inventors: Liu, Qiang; Sommer, Steve S.
Overview
Its claims, description and drawings are in the Canadian Patents Database.
Open the CIPO recordAbstract From the Canadian patent
A novel method of pyrophosphorolysis activated polymerization (PAP) has been developed. In PAP, pyrophosphorolysis and polymerization by DNA polymerase are coupled serially for each amplification by using an activatable oligonucleotide P* that has a non-extendable 3'-deoxynucleotide at its 3' terminus. PAP can be applied for exponential amplification or for linear amplification. PAP can be applied to amplification of a rare allele in admixture with one or more wild type alleles by using an activatable oligonucleotide P* that is an exact match at its 3' end for the rare allele but has a mismatch at or near its 3' terminus for the wild type allele. PAP is inhibited by a mismatch in …
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Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
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- Filed February 22, 2001 and granted August 2, 2011. A Canadian patent filed from October 1, 1989 lasts 20 years from filing, so its term ended February 22, 2021.
- A certificate of supplementary protection adds at most two years for a drug, so even that would have ended by February 22, 2023.
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Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.