Amplification based polymorphism detection

Abstract From the Canadian patent

Methods for detecting various types of polymorphic nucleic acid sequences are provided herein. The detection methods are based upon nucleic acid amplification procedures and the ability to detect "large" deletions or insertions in an automated fashion. For example a deletion or an insertion in , a target nucleic acid sequence in a test sample wherein the deletion or , insertion is at least 8 or more consecutive nucleotides can be detected , according to the following steps: a) contacting the test sample with amplification reagents and a set of amplification primers to form a reaction mixture wherein the set of amplification primers hybridize with the target nucleic acid sequence and a …