Oligonucleotides and assemblies thereof useful in the detection of the presence or absence of target nucleic acid sequences in a sample

Abstract From the Canadian patent

An oligonucleotide or assembly of oligonucleotides for the detection of the presence or absence of a target nucleic acid in a sample. The assembly of oligonucleotides comprises four regions a first and second regions which , hybridize to the target nucleic acid sequence and a third and fourth region where the third region is linked to the first region and the second region is linked to the fourth region. The third and fourth regions hybridize to one another. This hybridized structure can be cleaved with a restriction enzyme which cleavage will indicate hybridization of the oligonucleotide or oligonucleotides to the target. The invention also comprises methods of using these …