Embryonic or stem-like cell lines produced by cross species nuclear transplantation and methods for enhancing embryonic development by genetic alteration of donor cells or by tissue culture conditions

Abstract From the Canadian patent

An improved method of nuclear transfer involving the transplantation of differentiated donor cell nuclei into enucleated oocytes of a species different from the donor cell is provided. The resultant nuclear transfer units are useful for the production of isogenic embryonic stem cells in , particular human isogenic embryonic or stem cells. These embryonic or stemlike cells are useful for producing desired differentiated cells and for introduction, removal or modification, of desired genes e.g. at specific , sites of the genome of such cells by homologous recombination. These cells which may contain a heterologous gene, are especially useful in cell transplantation therapied and for in …