Methods of determining the presence of double stranded nucleic acids in a sample

Abstract From the Canadian patent

Methods for determining the presence of double stranded nucleic acids in a sample are provided. As illustrated in the figure, nucleic acids present in a fluid sample are translocated through a nanopore, e.g. by application of an electric field to the fluid sample. The current amplitude through the nanopore is monitored during the translocation process and changes in the amplitude are related to the passage of single- or double-stranded molecules through the nanopore. The subject methods find use in a variety of applications in which the detection of the presence of double-stranded nucleic acids in a sample is desired e.g. in , hybridization assays, such as Northern blot assays …