Method for sequencing nucleic acid molecules

Abstract From the Canadian patent

The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle the temporal , order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme on the templage nucleic acid molecule to be sequenced is followed in real time. The sequence is deduced by identifying which base is being incorporated into the growing complementary strand of the target nucleic acid by the catalytic activity of the nucleic acid polymerizing enzyme at each step in the sequence of base additions. A polymerase on the target nucleic acid molecule …