Improved methods for insertion of nucleic acids into circular vectors
CA 2355349 A1 · Owner: Romantchikov, Yuri · Inventors: Romantchikov, Yuri
Overview
Its claims, description and drawings are in the Canadian Patents Database.
Open the CIPO recordAbstract From the Canadian patent
The present invention provides vectors and methods which improve the efficiency of nucleic acid insertion into circular vectors which generally , facilitate nucleic acid cloning and specifically facilitate the preparation of DNA libraries. In general, the present invention involves separation of the cloning process into two distinct steps: (a) insertion which is done at a high nucleic acid concentration favoring intermolecular joining and (b) , circularization which is performed at a low nucleic acid concentration favoring intramolecular circularization. The present vectors generally have distinct insertion ends and circularization ends which are blocked from covalent joining during …
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Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Filed December 17, 1999. No grant is recorded for it in Canada; had it been granted, its 20-year term would have ended December 17, 2019.
- A certificate of supplementary protection adds at most two years for a drug, so even that would have ended by December 17, 2021.
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Timeline
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Sources & verification
Official CIPO records
Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.