Formulations and methods for isolating nucleic acids from any complex starting material and subsequent complex genetic analysis

Abstract From the Canadian patent

The invention relates to formulations without chaotropic components for isolating nucleic acids, notably DNA from any quantity of any complex starting material, by bonding to a solid phase. The formulations contain a lysis/bonding buffer system presenting at least one antichaotropic salt component, a solid phase and known washing and elution buffers. The lysis/bonding buffer system can be an aqueous solution or a solid formulation in ready-to-use reaction vessels. As the solid phase any support materials are suitable which are used for isolation by means of chaotropic reagents such , as, preferably, glass-fibre matting glass membranes, silicon supports, ceramic materials zeolites or …