Cell libraries indexed to nucleic acid microarrays

Abstract From the Canadian patent

This invention provides a method for selecting a clone of an ES cell containing a, mutation in a gene that is expressed in a test cell comprising: (a) providing cDNA, obtained by reverse transcription of mRNA of the test cell; (b) providing a collection of, cultured ES cells organized into individual clones wherein each clone is of , an ES cell, having a mutation in an exon in its genome the mutation being in a different , exon in cells of different clones; (c) providing an array of different single stranded polynucleotides, the, polynucleotides being fragments of exons containing mutations in (b); (d) exposing the, cDNA to the array under conditions permitting hybridization of …