Expression and export of angiogenesis inhibitors as immunofusins

Abstract From the Canadian patent

Disclosed are nucleotide sequences, for example DNA or RNA sequences, which , encode an immunoglobulin Fc-angiogenesis inhibitor fusion protein. The angiogenesis inhibitors can be angiostatin endostatin, a plasminogen fragment having angiostatin activity or a collagen XVIII fragment having endostatin activity. The nucleotide sequences can be inserted into a suitable expression vector and expressed in mammalian cells. Also disclosed is a family of immunoglobulin Fcangiogenesis inhibitor fusion proteins that can be produced by expression of such nucleotide sequences. Also disclosed are methods using such nucleotide sequences and fusion proteins for treating conditions mediated by …