.alpha.2,8/2,9 polysialyltransferase
CA 2339173 A1 · Owner: Datta, Arun · Inventors: Wong, Chi-Huey; Datta, Arun; Shen, Gwo-Jenn
Overview
Its claims, description and drawings are in the Canadian Patents Database.
Open the CIPO recordAbstract From the Canadian patent
The capsular polysaccharide of Escherichia coli K92 contains alternating (-8- NeuAc.alpha.2-) and (-9-NeuAc.alpha.2-) linkages. The enzyme catalyzing this polymerizing reaction has been cloned from the genomic DNA of E. coli K92. The 1.2 kb PCR1 fragment was subcloned in pRSET vector and the protein was expressed in the BL21(DE3) strain of E. coli with a hexameric histidine at its N-terminal end. The enzyme was isolated in the supernatant after lysis of the cells and fractionated by ultracentrifugation. Western blotting using antihistidine antibody showed the presence of a band that migrated at about 47.5 kD on both reducing and non-reducing SDS-PAGE indicating a monomeric enzyme. , …
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Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Filed August 10, 1999. No grant is recorded for it in Canada; had it been granted, its 20-year term would have ended August 10, 2019.
- A certificate of supplementary protection adds at most two years for a drug, so even that would have ended by August 10, 2021.
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Timeline
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Sources & verification
Official CIPO records
Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.