Assay using porosity-reduction to inhibit migration

Abstract From the Canadian patent

Device for assaying an analyte, comprising a labelling zone where a label can , bind to the analyte in communication with a capture zone, wherein the pore size of the capture zone is such that label which is not bound to the analyte can migrate therethrough, whereas label which is bound to the analyte cannot. During migration from the labelling zone (large pore size) to the capture zone (small pore size), unbound label can pass into and through the capture zone whereas bound label will be captured at the junction of the labelling zone and the capture zone. The device relies upon the label being smaller than the analyte, such that free label is not retarded by the capture zone. It is …