In situ hybridization method for detecting target nucleic acid
CA 2332510 C · Owner: Shah, Jyotsna S. · Inventors: Shah, Jyotsna S.; Harris, Nick S.
Overview
Its claims, description and drawings are in the Canadian Patents Database.
Open the CIPO recordAbstract From the Canadian patent
The present invention provides a method for detecting a target nucleic acid fragment directly from a specimen obtained from a patient by in situ hybridization. The method is comprised of several steps which are performed in the listed order. A sample of the specimen is deposited onto a slide. The sample is fixed onto the slide with fixative the fixative comprising either , methanol-acetic acid at a ratio of from 99:1 to 80:20 or formalin-acetic acid , at a ratio of from 99:1 to 80:20. The nucleic acids of the fixed sample are contacted with a probe complex specific for the target nucleic acid fragment under conditions appropriate for hybridization. Non-hybridized probe complex is …
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Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Filed May 18, 1999 and granted July 26, 2011. A Canadian patent filed from October 1, 1989 lasts 20 years from filing, so its term ended May 18, 2019.
- A certificate of supplementary protection adds at most two years for a drug, so even that would have ended by May 18, 2021.
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Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.