Expression of functional eukaryotic proteins
CA 2331777 A1 · Owner: Lin, Zhanglin · Inventors: Lin, Zhanglin; Arnold, Frances H.
Overview
Its claims, description and drawings are in the Canadian Patents Database.
Open the CIPO recordAbstract From the Canadian patent
This invention relates to the improved expression of evolved polynucleotide and polypeptide sequences encoding for eukaryotic enzymes particularly , peroxidase enzymes, in conventional or facile expression systems. Various methods for directed evolution of polynucleotide sequences can be used to obtain the improved sequences. The improved characteristics of the polypeptides or proteins generated in this manner include improved folding without formation of inclusion bodies, and retained functional activity. In a particular embodiment, the invention relates to improved expression of the horseradish peroxidase gene and horseradish peroxidase enzymes.
Read the whole patent
Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Filed July 28, 1999. No grant is recorded for it in Canada; had it been granted, its 20-year term would have ended July 28, 2019.
- A certificate of supplementary protection adds at most two years for a drug, so even that would have ended by July 28, 2021.
- We check Canadian rights only. A patent in its family in another country could still be in force: check its family on Google Patents.
Timeline
Timeline From CIPO dates
Sources & verification
Official CIPO records
Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.