Method for the isolation of ccc plasmid dna
CA 2329246 A1 · Owner: Friehs, Karl · Inventors: Flaschel, Erwin; Friehs, Karl; Schmidt, Torsten
Overview
Its claims, description and drawings are in the Canadian Patents Database.
Open the CIPO recordAbstract From the Canadian patent
The present invention relates to the production of biomass for the isolation of ccc plasmid DNA comprising culturing a bacterial transformant in a bioreactor containing an antibiotic-free batch medium under batch-conditions and, at the end of the batch phase, feeding under feed-back conditions the portion of a feed-back medium after the rise of DO above a threshold-set point. Said feed-back medium comprises besides a carbon source a magnesium salt, preferably in concentrations above 20 mM. Preferably the bacterial , transformant is harvested after the end of the culture and frozen or freezedried. Also preferred is that ccc plasmid DNA is optionally directly, isolated after harvesting …
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Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Filed May 21, 1999. No grant is recorded for it in Canada; had it been granted, its 20-year term would have ended May 21, 2019.
- A certificate of supplementary protection adds at most two years for a drug, so even that would have ended by May 21, 2021.
- We check Canadian rights only. A patent in its family in another country could still be in force: check its family on Google Patents.
Timeline
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Sources & verification
Official CIPO records
Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.