Hybridization and mismatch discrimination using oligonucleotides conjugated to minor groove binders
CA 2329135 C · Owner: Belousov, Evgeniy S. · Inventors: Afonina, Irina A.; Meyer, Rich B. Jr.; Kutyavin, Igor V.
Overview
Its claims, description and drawings are in the Canadian Patents Database.
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Conjugates between a minor groove binding molecule such as the trimer of 1,2-, dihydro-(3H)-pyrrolo3,2-eindole-7-carboxylate (CDPI3) and an , oligonucleotide form unusually stable hybrids with complementary target sequences, in which the tethered CDPI3 group resides in the minor groove of the duplex. These conjugates can be used as probes and primers. Due to their unusually high binding affinity, conjugates as short as 8-mers can be used as amplification primers with high specificity and efficiency. MGB conjugation also increases the discriminatory power of short oligonucleotides providing , enhanced detection of nucleotide sequence mismatches by short oligonucleotides. The …
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Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Filed April 5, 1999 and granted May 27, 2014. A Canadian patent filed from October 1, 1989 lasts 20 years from filing, so its term ended April 5, 2019.
- A certificate of supplementary protection adds at most two years for a drug, so even that would have ended by April 5, 2021.
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